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KMID : 0370220100540010008
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Yakhak Hoeji
2010 Volume.54 No. 1 p.8 ~ p.12
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Knock-down of Type 2 Inositol 1,4,5-Trisphosphate Receptors using Adenovirus in Adult Ventricular Myocytes
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Son Min-Jeong
Krishna P. Subedi
Woo Sun-Hee
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Abstract
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Inositol 1,4,5-trisphosphate () receptor ()-mediated signaling pathway is involved in many cellular processes including fertilization, apoptosis and neuronal function. Although cardiac myocytes express the , its pathophysiological role has not been clearly understood because of limited selectivity of currently available pharmacological blockers. In the present study we constructed shRNA-expressing adenovirus to knock-down the type 2 (), a major subtype in cardiac ventricular myocytes, and demonstrated that the virus successfully eliminated the expression and localization of the . These results may provide a reliable tool for probing pathophysiological roles of the in isolated intact cardiac myocytes.
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KEYWORD
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inositol 1, 4, 5-trisphosphate receptor (IP3R), adenoviral knock-down, adult ventricular myocytes
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